研究成果 | 科研项目 1. 国家重点研发计划项目（课题）（2023YFF1000903），负责人。 2. 国家肉羊产业技术体系项目 （CARS38），岗位科学家。 发表文章 1. An Q, Chen T, Jiang J, Wu H, Wu G, Jiao Z, Li S, Meng Y, Tang J, Chen Y, Pan H, Li H, Zhang Z, Cheng Y, Fu Y, Huang Z, Man C, Chen Q*, Wang F*. Profiling cell dynamic changes of goat peripheral blood mononuclear cells after Pasteurella multocida infection with single-cell transcriptomics and histopathology. Veterinary Research. Accepted. 2. Yang Y, Chen X, Wang Z, Wu L, Du L, Gao H, Chen S, Chen Q*, Wang F*. Dysregulated interferon signaling and hyperinflammation upon FCGBP knockdown in goat bronchial epithelial cells infected with Pasteurella multocida. Frontiers in Veterinary Science. 2026, 13: 1761935. Doi: 10.3389/fvets.2026.1761935. 3. Li S, Xu Le, Jiao Y, Li S, Yang Y, Lan F, Chen Si, Man C, Du L, Chen Q, Wang F*, Gao H*. Risk assessment of global animal melioidosis under current and future climate scenarios, Animals. 2025, 15(3): 455. Doi: 10.3390/ani15030455. 4. Meng Y, Zhang W, Cheng Y, Wu Y, Wu H, He M, Chen S, Man C, Gao H, Du L, Chen Q*, Wang F*. Development and verification of a 10K liquid chip for Hainan black goat based on genotyping by pinpoint sequencing of liquid captured targets. BMC Genomic Data. 2024, 25(1): 44. Doi: 10.1186/s12863-024-01228-8. 5. Pan H, Jiao Z, Li H, Li S, Xu L, Li S, Meng Y, Fu Y, Chen T, Chen Q, Chen S, Du L, Man C, Wang F*, Gao H*. Sheep and goat pathogen database: a pathogen data integration and analysis database of sheep and goat infectious diseases. Frontiers in Microbiology. 2024, 14: 1299303. Doi: 10.3389/fmicb.2023.1299303. 6. Wang H, Wu H, Zhang W, Jiang J, Qian H, Man C, Gao H, Chen Q, Du L, Chen S*, Wang F*. Development and validation of a 5K low-density SNP chip for Hainan cattle. BMC Genomics. 2024, 25(1): 873. Doi: 10.1186/s12864-024-10753-w. 7. Chai Y, Li S, Wu H, Meng Y, Fu Y, Li H, Wu G, Jiang J, Chen T, Jiao Y, Chen Q, Du L, Li L, Man C, Chen S, Gao H*, Zhang W*, Wang F*. The genome landscape of the Xinglong buffalo. BMC Genomics. 2024, 25(1): 1054. Doi: 10.1186/s12864-024-10941-8. 8. Yang X, Fu Q, Zhang W, An Q, Zhang Z, Li H, Chen X, Chen Z, Cheng Y, Chen S, Man C, Du L*, Chen Q*, Wang F*. Overexpression of Pasteurella multocida OmpA induces transcriptional changes and its possible implications for the macrophage polarization. Microbial Pathogenesis. 2023, 183: 106212. Doi: 10.1016/j.micpath.2023.106212. 9. Chen S, Chang C, Cui K, Yang W, Li B, Ni S, Zhang W, Li S, Li X, Wu G, Li L, Chen Q, Man C, Du L, Zhang W*, Wang F*. Whole-genome analyses reveal the genomic diversity and selection signatures of Hainan cattle. Livestock Science. 2023, 275: 105311. Doi: 10.1016/j.livsci.2023.105311. 10. Chen Q, Chen Z, Zhang Z, Pan H, Li H, Li X, An Q, Cheng Y, Chen S, Man C, Du L*, Wang F*. Profiling chromatin accessibility responses in goat bronchial epithelial cells infected with Pasteurella multocida. International Journal of Molecular Sciences. 2023, 24(2): 1312. Doi: 10.3390/ijms24021312. 11. Chen S, Chen Y, Jiao Z, Wang C, Zhao D, Liu Y, Zhang W, Zhao S, Yang B, Zhao Q, Fu S, He X, Chen Q, Man C, Liu G, Wei X, Du L*, Wang F*. Clearance of bacteria from lymph nodes in sheep immunized with Brucella suis S2 vaccine is associated with M1 macrophage activation. Veterinary Research. 2023, 54(1): 20. Doi: 10.1186/s13567-023-01147-z. 12. He M, Li H, Zhang Z, Jiang J, Li H, Yang W, Cheng Y, Gao H, Chen Q, Du L, Chen S*, Man C, Wang F*. Microbiological characteristics and pathogenesis of Klebsiella pneumoniae isolated from Hainan Black Goat. Veterinary Sciences. 2022, 9(9): 471. Doi: 10.3390/vetsci9090471. 13. Li L, Li K, Bian Z, Chen Z, Li B, Cui K, Wang F*. Association between body weight and distal gut microbes in Hainan Black Goats at weaning age. Frontiers in Microbiology. 2022, 13: 951473. Doi: 10.3389/fmicb.2022.951473. 14. Zhang Z, Jiang J, He M, Li H, Cheng Y, An Q, Chen S, Du L, Man C, Chen Q*, Li L*, Wang F*. First report and comparative genomic analysis of a Mycoplasma mycoides Subspecies capri HN-A in Hainan Island. Microorganisms 2022,10,1908. Doi:10.3390/microorganisms10101908. 15. Zhang Z, He M, Jiang J, Li X, Li H, Zhang W, Chen S, Du L, Man C, Chen Q*, Gao H*, Wang F*. First report and comparative genomic analysis of Mycoplasma capricolum Subsp.capricolum HN-B in Hainan Island, China. Microorganisms.2022,10, 2298. Doi:10.3390/microorganisms10112298. 16. Chen Q, Chai Y, Zhang W, Cheng Y, Zhang Z, An Q, Chen S, Man C, Du L, Zhang W*, Wang F*. Whole-genome sequencing reveals the genomic characteristics and selection signatures of Hainan Black Goat. Genes. 2022, 13(9): 1539. Doi: 10.3390/genes13091539. 17. Zhang X, Cui K, Wen X, Li L, Yu X, Li B, Lin H, He H*, Wang F*. The association between gut microbiome diversity and composition and heat tolerance in cattle. Microorganisms. 2022, 10(8): 1672. Doi: 10.3390/microorganisms10081672. 18. Chen S, Wang C, Chen Q, Zhao D, Liu Y, Zhao S, Fu S, He X, Yang B, Zhao Q, An Q, Zhang Z, Cheng Y, Man C, Liu G, Wei X, Zhang W*, Du L*, Wang F*. Downregulation of three novel miRNAs in the lymph nodes of sheep immunized with the Brucella suis strain 2 vaccine. Frontiers in Veterinary Science. 2022, 9: 813170. Doi: 10.3389/fvets.2022.813170. 19. Chen Q, Zhang Z, Chen S, Chen J, Cheng Y, Liu A, Li B, Chen Z, Zheng Y, Ga M, Du L, Wang F*. Genome-wide differential expression profiling of pulmonary circrnas associated with immune reaction to Pasteurella multocida in goats. Frontiers in Veterinary Science. 2021, 8: 615405. Doi: 10.3389/fvets.2021.615405. 20. Jiao H, Zhao Y, Zhou Z, Li W, Li B, Gu G, Luo Y, Shuai X, Fan C, Wu L, Chen J, Huang Q, Wang F*, Liu J*. Identifying circular RNAs in HepG2 expressing genotype IV swine hepatitis E virus ORF3 via whole genome sequencing. Cell Transplant. 2021, 30: 9636897211055042. Doi: 10.1177/09636897211055042. 21. Li B, Chen S, Wang C, Chen Q, Man C, An Q, Zhang Z, Liu Z, Du L*, Wang F*. Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to Brucella Melitensis strain M5-90. PeerJ. 2021, 9: e11679. Doi: 10.7717/peerj.11679. 22. Pang F, Wang X, Chen Z, Zhang Z, Zhang M, Wang C, Yang X, An Q, Du L, Wang F*. Integrated analysis of differentially expressed miRNAs and mRNAs in goat skin fibroblast cells in response to Orf virus infection reveals that cfa-let-7a regulates thrombospondin 1 expression. Viruses. 2020, 12(1): 118. Doi: 10.3390/v12010118. 23. Jiao H, Nie X, Zhu H, Li B, Pang F, Yang X, Cao R, Yang X, Zhu S, Peng D, Li Y, Li G, Zhang Z, Huang H, Xu K, Zhao T, Cheng Y, Chen C*, Du L*, Wang F*. miR-146b-5p plays a critical role in the regulation of autophagy in ∆per Brucella melitensis-infected RAW264.7 cells. BioMed Research International. 2020, 2020:1953242. Doi: 10.1155/2020/1953242. 24. Pang F, Chen Z, Wang C, Zhang M, Zhang Z, Yang X, Zheng Y, Liu A, Cheng Y, Chen J, Li B, Du L, Wang F*. Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene. Peer J. 2019, 7: e8098. Doi: 10.7717/peerj.8098. 25. Pang F, Zhang M, Li G, Zhang Z, Huang H, Li B, Wang C, Yang X, Zheng Y, An Q, Zhang L, Du L, Wang F*. Integrated mRNA and miRNA profiling in NIH/3T3 cells in response to bovine papillomavirus E6 gene expression. Peer J. 2019, 7: e7442. Doi: 10.7717/peerj.7442. 26. Pang F, Zhang M, Yang X, Li G, Zhu S, Nie X, Cao R, Yang X, Zhang Z, Huang H, Li B, Wang C, Du L, Wang F*. Genome-wide analysis of circular RNAs in goat skin fibroblast cells in response to Orf virus infection. Peer J. 2019, 7: e6267. Doi: 10.7717/peerj.6267. 27. Zhu H, Jiao H, Nie X, Li B, Xu K, Pang F, Cao R, Zhu S, Yang X, Zhang Z, Peng D, Li Y, Li G, Huang H, Chen C*, Du L*, Wang F*. Alterations of microRNAs and their predicted targeting mRNAs expression in RAW264.7 macrophages infected with Omp25 mutant Brucella melitensis. Innate Immunity. 2018, 24(6): 382-389. Doi: 10.1177/1753425918792298. 28. Peng D, Pang F, Cao R, Zhu S, Yang X, Nie X, Zhang Z, Li B, Huang H, Li Y, Li G, Du L, Wang F*. Upregulation of immune process-associated genes in RAW264.7 macrophage cells in response to Burkholderia pseudomallei Infection. BioMed Research International. 2018, 2018: 1235097. Doi: 10.1155/2018/1235097. 29. Rong H, Jiao H, Hao Y, Pang F, Li G, Peng D, Li Y, Wang Y, Zhang H, Fan Q, Wang F*, Chen C*, Du L*. CD14 gene silencing alters the microRNA expression profile of RAW264.7 cells stimulated by Brucella melitensis infection. Innate Immunity. 2017, 23(5): 424-431. Doi: 10.1177/1753425917707025. 30. Xu K, Guo S, Zhao T, Zhu H, Jiao H, Shi Q, Pang F, Li Y, Li G, Peng D, Nie X, Cheng Y, Wu K, Du L, Cui K, Zhang W*, Wang F*. Transcriptome analysis of HepG2 cells expressing ORF3 from swine hepatitis E virus to determine the effects of ORF3 on host cells. BioMed Research International. 2016, 2016: 1648030. Doi: 10.1155/2016/1648030. 31. Jiao H, Jia X, Zhao T, Rong H, Zhang J, Cheng Y, Zhu H, Xu K, Guo S, Shi Q, Zhang H, Wang F*, Chen C*, Du L*. Up-regulation of TDAG51 is a dependent factor of LPS-induced RAW264.7 macrophages proliferation and cell cycle progression. Immunopharmacology And Immunotoxicology. 2016, 38(2): 124-130. Doi: 10.3109/08923973.2016.1138968. 32. Chen H, Jiao H, Cheng Y, Xu K, Jia X, Shi Q, Guo S, Wang M, Du L, Wang F*. In Vitro and In Vivo immunomodulatory activity of Okra (Abelmoschus esculentus L.) polysaccharides. Journal of Medicinal Food. 2016, 19(3): 253-265. Doi: 10.1089/jmf.2015.3513. 33. Cheng Y, Du L, Jiao H, Zhu H, Xu K, Guo S, Shi Q, Zhao T, Pang F, Jia X, Wang F*. Mmu-miR-27a-5p-dependent upregulation of MCPIP1 inhibits the inflammatory response in LPS-induced RAW264.7 macrophage cells. BioMed Research International. 2015, 2015: 607692. Doi: 10.1155/2015/607692. 34. Pang F, Shi Q, Du L, Zhao T, Cheng Y, Jiao H, Zhao J, Wang M, Rong H, Zhou H, Wang F*. Complete genome sequence of bovine papillomavirus genotype 13 from local yellow cattle in Hainan Province, China. Genome Announcements. 2014, 2(6): e01087-14. Doi: 10.1128/genomeA.01087-14. 35. Du L, Rong H, Cheng Y, Guo S, Shi Q, Jia X, Zhu H, Hao Y, Xu K, Zhang J, Jiao H, Zhao T, Zhang H, Chen C, Wang F*. Identification of microRNAs dysregulated in CD14 gene silencing RAW264.7 macrophage cells. Inflammation. 2014, 37(1): 287-294. Doi: 10.1007/s10753-013-9739-3. 36. Cheng Y, Du L, Shi Q, Jiao H, Zhang X, Hao Y, Rong H, Zhang J, Jia X, Guo S, Kuang W, Zhang H, Chen C, Wang F*. Identification of miR-221 and -222 as important regulators in genotype IV swine hepatitis E virus ORF3-expressing HEK 293 cells. Virus Genes. 2013, 47(1): 49-55. Doi: 10.1007/s11262-013-0912-4. 37. Jiao H, Du L, Hao Y, Cheng Y, Luo J, Kuang W, Zhang D, Lei M, Jia X, Zhang X, Qi C, He H*, Wang F*. Effective inhibition of mRNA accumulation and protein expression of H5N1 avian influenza virus NS1 gene in vitro by small interfering RNAs. Folia Microbiologica. 2013, 58(4): 335-342. Doi: 10.1007/s12223-012-0212-8. 38. Cheng Y, Kuang W, Hao Y, Zhang D, Lei M, Du L, Jiao H, Zhang X, Wang F*. Downregulation of miR-27a* and miR-532-5p and upregulation of miR-146a and miR-155 in LPS-induced RAW264.7 macrophage cells. Inflammation. 2012, 35(4): 1308-1313. Doi: 10.1007/s10753-012-9443-8. 39. Lei M, Du L, Jiao H, Cheng Y, Zhang D, Hao Y, Li G, Qiu W, Fan Q, Li C, Chen C*, Wang F*. Inhibition of mCD14 inhibits TNFα secretion and NO production in RAW264.7 cells stimulated by Brucella melitensis infection. Veterinary Microbiology. 2012, 160(3-4): 362-368. Doi: 10.1016/j.vetmic.2012.05.039. 40. Lei M, Jiao H, Liu T, Du L, Cheng Y, Zhang D, Hao Y, Man C, Wang F*. siRNA targeting mCD14 inhibits TNF-α, MIP-2, and IL-6 secretion and NO production from LPS-induced RAW264.7 cells. Applied Microbiology and Biotechnology. 2011, 92(1): 115-124. Doi: 10.1007/s00253-011-3371-7. 41. Liu T, Lei M, Jiao H, Du L, Cheng Y, Zhang D, Hao Y, Man C, Wang F*. RNA interference induces effective inhibition of mRNA accumulation and protein expression of SHEV ORF3 gene in vitro. Current Microbiology. 2011, 62(5): 1355-1362. Doi: 10.1007/s00284-010-9863-3. 出版著作 1. 《兽医微生物学》，科学出版社，2007，主译。 2. 《细菌分子遗传学》，科学出版社，2013，主译。 3. 《畜牧微生物学》（第五版），中国农业出版社，2009，参编。 4. 《畜牧微生物学》（第六版），中国农业出版社，2017，参编。 5. 《绵羊与山羊医学》（第二版），中国农业大学出版社，2020，主译。 6. 《畜禽种业科技创新战略研究》，科学出版社，2024，编委。 |
